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X‐ray absorption studies on bacteriorhodopsin
Author(s) -
Engelhard M.,
Hess B.,
Chance M.,
Chance B.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80385-x
Subject(s) - bacteriorhodopsin , extended x ray absorption fine structure , chemistry , absorption (acoustics) , crystallography , homogeneous , photochemistry , absorption spectroscopy , membrane , optics , physics , biochemistry , thermodynamics
Extended X‐ray absorption (EXAFS) measurements of iron‐substituted bacteriorhodopsin revealed for the light‐adapted state (bR LA ) a homogeneous distribution of the first‐shell ligands of iron. In Contrary to this result, the dark‐adapted (bR DA ) and the M‐state (bR M ) display significant heterogeneity at the iron site with bR DA intermediate between bR M and bR LA in average distance. These data provide direct evidence of structural changes at the membrane associated with photocycle changes at the cation‐binding site.