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Epidermal and transforming growth factors modulate secretion of a 69 kDa phosphoprotein in normal rat kidney fibroblasts
Author(s) -
Laverdure G.R.,
Banerjee D.,
Chackalaparampil I.,
Mukherjee B.B.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80382-4
Subject(s) - phosphoprotein , secretion , transforming growth factor , microbiology and biotechnology , epidermal growth factor , kidney , chemistry , medicine , endocrinology , biology , phosphorylation , biochemistry , receptor
Our study shows that the secretion of a major non‐glycosylated, phosphoprotein of 69 kDa (pp69) is a specific marker for non‐transformed NRK‐49F cells. Treatment of NRK‐49F cells with EGF alone or with different combinations of EGF plus TGF‐β modulates the secretion of pp69, suggesting its relationship with cellular proliferation. Antibody raised against pp69 recognizes, in addition to pp69, another major phosphoprotein of 62 kDa (pp62) secreted by RR1022 and spontaneously transformed NRK‐49F cells. Immunoprecipitation of total cell lysates from both NRK‐49F and RR1022 cells with anti‐pp69 antibody detected only pp69. These observations suggest a precursor‐product relationship between pp69 secreted by non‐transformed NRK‐49F cells and pp62 secreted by transformed cells.