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Is there a root effect in Xenopus hemoglobin?
Author(s) -
Brunori Maurizio,
Bellelli Andrea,
Giardina Bruno,
Condo Saverio,
Perutz Max F.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80372-1
Subject(s) - xenopus , bohr effect , tetramer , hemoglobin , chemistry , biophysics , oxygen , carbon monoxide , stereochemistry , biochemistry , biology , organic chemistry , enzyme , oxygen–haemoglobin dissociation curve , gene , catalysis
The reaction of Xenopus hemoglobin with oxygen and carbon monoxide has been reinvestigated over the pH range 8.5–6.0, in the absence and presence of organic phosphates (2,3‐diphosphoglycerate or inositol hexakisphosphate), to establish if the tetramer can be stabilized in a T‐quaternary state by protons and polyphosphate; the equilibrium and kinetic data indicate that Xenopus hemoglobin does exhibit a Root effect. These new results are discussed with reference to those reported by Bridges et al. [(1985) Resp. Physiol. 61, 125‐136] on Xenopus blood and, more generally, to the molecular definition and the structural basis of the Root effect as an extreme form of the Bohr effect.