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Activation and inhibition of the calcium gate of sarcoplasmic reticulum by high‐affinity ryanodine binding
Author(s) -
Hasselbach Wilhelm,
Migala Andrea
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80364-2
Subject(s) - ryanodine receptor , calcium , caffeine , chemistry , ryanodine receptor 2 , endoplasmic reticulum , biophysics , biochemistry , endocrinology , biology , organic chemistry
The occupancy of high‐affinity ryanodine‐binding sites of isolated heavy sarcoplasmic reticulum vesicles occurring in concentrated salt solutions affects ATP‐dependent calcium accumulation and caffeine‐induced calcium release. The initial suppression of calcium uptake is followed by a marked uptake activation resulting in a reduction of the final calcium level in the medium. Simultaneously, caffeine‐induced calcium release is blocked. The dependence of inhibition of calcium uptake and caffeine‐induced calcium release observed in assay media containing physiological concentrations of magnesium and ATP on the concentration of ryanodine corresponds to the drug's effectiveness in living muscles.