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Recycling of epidermal growth factor in A431 cells
Author(s) -
Teslenko L.V.,
Kornilova E.S.,
Sorkin A.D.,
Nikolsky N.N.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80361-7
Subject(s) - a431 cells , epidermal growth factor , chemistry , factor (programming language) , microbiology and biotechnology , biology , biochemistry , computer science , cell , cell cycle , programming language , molecular medicine , receptor
The fate of epidermal growth factor (EGF) after internalization by A431 cells was studied. First, cells containing 125 I‐EGF‐receptor complexes in endosomes were obtained. Subsequent incubation of the cells at 37°C resulted in the recycling of 125 I‐EGF from endosomes to the cell surface in the receptor‐bound state and the gradual release of recycled ligand into the medium. The excess of unlabeled EGF blocked both rebinding and re‐internalization of recycled 125 I‐EGF to produce enhanced accumulation of ligand in the medium. The rate of recycling was shown to be much higher than that of EGF degradation.