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Recombinant γ‐interferon stimulates iodide uptake and cyclic AMP production by the FTRL 5 thyroid cell line
Author(s) -
Weetman A.P.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80358-7
Subject(s) - cycloheximide , recombinant dna , cell culture , in vitro , iodide , endocrinology , thyroid , medicine , chemistry , interferon , cell growth , cell , protein biosynthesis , biochemistry , biology , microbiology and biotechnology , immunology , genetics , gene , organic chemistry
The effect of recombinant rat γ‐interferon (γ‐IFN) on iodide uptake and cAMP production by rat thyroid cells in vitro was studied using the continuously growing, functional FRTL 5 cell line. Both functions were stimulated by γ‐IFN at concentrations of 1–10 U/ml. Iodide uptake was dependent on protein synthesis, since it was blocked by cycloheximide treatment, but was not dependent on growth factors in calf serum routinely used for FRTL 5 cell culture. These results show that γ‐IFN can stimulate thyroid cell function as well as aberrant Ia expression in vitro.