Premium
Synthetic diacylglycerols induce a rise of quin2‐detectable free intracellular calcium in human platelets
Author(s) -
Bruns Christian,
Schächtele Christoph,
Marmé Dieter
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80345-9
Subject(s) - protein kinase c , forskolin , intracellular , phorbol , chemistry , platelet , cytoplasm , calcium , calcium in biology , biochemistry , diacylglycerol kinase , fura 2 , biophysics , enzyme , biology , cytosol , in vitro , organic chemistry , immunology
The two activators of protein kinase C, oleoylacetylglycerol (OAG) and dioctanoylglycerol (DOG), are able to induce a concentration‐dependent rise in cytoplasmic free Ca 2+ concentration in gel‐filtered human platelets, detected as an increase in quin2 fluorescence. The phorbol ester phorbol‐12‐myristate‐13‐acetate (PMA) has no effect. The OAG‐induced increase of intracellular Ca 2+ is not influenced by forskolin, in contrast to the effect of the diterpene on the thrombin‐stimulated increase in cytoplasmic Ca 2+ . It is concluded that the increase in intracellular free Ca 2+ concentration induced by synthetic diacylglycerols and their activation of protein kinase C are two different and independent processes.