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Increase of DPH fluorescence polarization during development of Dictyostelium discoideum cells
Author(s) -
Thiery R.,
Klein R.,
Tatischeff I.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80323-x
Subject(s) - dictyostelium discoideum , diphenylhexatriene , fluorescence anisotropy , fluorescence , biophysics , chemistry , membrane , microbiology and biotechnology , biochemistry , biology , optics , physics , gene
Whole cells of the eukaryotic microorganism Dictyostelium discoideum have been assayed with membrane fluorescence polarization probes. With diphenylhexatriene (DPH) the steady‐state fluorescence polarization remained stable during growth, but significant rise was found as a function of time of development. This variation, discussed as being specific to the plasma membrane, is the first report of a biophysical change occurring at this level during cellular aggregation of D. discoideum . In contrast, with trimethylammonium (TMA)‐DPH no change was observed during development. The differences obtained with the two probes are discussed in the light of complementary fluorescence anisotropy decay measurements.