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Identifying subunits of ATP synthase TF 0 ·F 1 in contact with phospholipid head groups α‐Subunits are labelled selectively by a new photoreactive phospholipid designed for hydrophilic photolabelling
Author(s) -
Gao Zhan,
Bäuerlein Edmund
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80320-4
Subject(s) - phospholipid , thermophile , phosphatidylethanolamine , atp synthase , liposome , ethanolamine , chemistry , biochemistry , amphiphile , enzyme , phosphatidylcholine , membrane , organic chemistry , polymer , copolymer
A new phospholipid photolabel was introduced by modifying 1,2‐dipalmitoylphosphatidylethanolamine with 4‐azidosalicylate to 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phospho‐ N ‐(4‐azido‐2‐hydroxybenzoyl)ethanolamine (ASA‐PE), which could be radioiodinated easily to 125 I‐ASA‐PE. The ATP synthase TF 0 ·F 1 of the thermophilic bacterium PS3 was reconstituted with soybean phospholipids forming proteoliposomes with high ATP‐ 32 P i exchange activity. These proteoliposomes were incubated with 125 I‐ASA‐PE to provide its selective incorporation into the outside of the phospholipid bilayer. Upon illumination with ultraviolet light α‐subunits of TF 1 were predominantly labelled.