Premium
Fate of polyoma origin of replication after its direct introduction into mice
Author(s) -
Cohen Hannah,
Benvenisty Nissim,
Reshef Lea
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80317-4
Subject(s) - plasmid , spleen , dna replication , biology , dna , replication (statistics) , origin of replication , microbiology and biotechnology , virology , genetics , immunology
Recently we have developed a method for direct introduction of calcium phosphate‐precipitated DNA into newborn rats. To examine whether the foreign DNA can replicate, a plasmid containing a polyoma origin of replication was injected into newborn mice. The plasmid was found intact in liver and spleen and able to transform bacteria. The foreign DNA had disappeared by the seventh day after injection. Yet, the plasmid DNA containing the polyoma origin of replication had undergone replication in both the liver and the spleen.