Premium
Isolation and sequence analysis of a cDNA clone encoding a type‐1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2A
Author(s) -
Berndt Norbert,
Campbell David G.,
Caudwell F.Barry,
Cohen Philip,
da Cruz e Silva Edgar F.,
da Cruz e Silva Odete B.,
Cohen Patricia T.W.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80316-2
Subject(s) - complementary dna , protein subunit , microbiology and biotechnology , phosphatase , dusp6 , homology (biology) , protein phosphatase 2 , sequence analysis , biology , protein phosphatase 1 , nucleic acid sequence , genetics , biochemistry , enzyme , gene
A 1.5 kb clone containing the full‐length coding sequence of a type‐1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type‐1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1–101 of the type‐1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.