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Cross‐reconstitution of isolated F 1 ‐ATPase from potato tuber mitochondria with F 1 ‐depleted beef heart and yeast submitochondrial particles
Author(s) -
Norling B.,
Hamasur B.,
Glaser E.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80310-1
Subject(s) - submitochondrial particle , oligomycin , yeast , atpase , heterologous , mitochondrion , membrane , chemistry , biochemistry , biology , enzyme , gene
Cross‐reconstitution of isolated potato mitochondrial F 1 ‐ATPase with F 1 ‐depleted beef heart and yeast submitochondrial particles is reported. Potato F 1 binds to the heterologous membrane and confers oligomycin sensitivity on the ATPase activity of the reconstituted system. Binding of F 1 is promoted by the presence of Mg 2+ with the maximal stimulatory efrect at 20 mM. Mg 2+ increase the sensitivity to oligomycin of the reconstituted system consisting of potato F 1 and yeast membranes, however, they do not influence oligomycin sensitivity of potato F 1 and beef heart membranes.