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The long 3′‐untranslated regions of the PDGF‐A and ‐B mRNAs are only distantly related
Author(s) -
Hoppe J.,
Schumacher L.,
Eichner W.,
Weich H.A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80297-1
Subject(s) - untranslated region , complementary dna , coding region , clone (java method) , microbiology and biotechnology , biology , messenger rna , oligonucleotide , three prime untranslated region , platelet derived growth factor receptor , oligomer restriction , nucleic acid sequence , genetics , gene , growth factor , receptor
A cDNA clone of about 2300 base pairs was prepared from the human osteosarcoma cell line U‐2 OS by hybridization with a 22‐mer oligonucleotide complementary to the NH 2 ‐terminus of PDGF‐A. Restriction and sequence analysis showed that this clone contains the entire coding region for PDGF‐A and a long 3′‐untranslated region which is only distantly related to that in the mRNA of PDGF‐B.