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Progressin: A novel proliferation‐sensitive and cell cycle‐regulated human protein whose rate of synthesis increases at or near the G 1 /S transition border of the cell cycle
Author(s) -
Celis Julio E.,
Ratz Gitte Petersen,
Celis Ariana
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80296-x
Subject(s) - cell cycle , dna synthesis , dna replication , cell division , microbiology and biotechnology , fibroblast , biology , cell growth , histone , s phase , cell , dna , cell culture , eukaryotic dna replication , genetics
A novel proliferation‐sensitive and cell cycle‐specific basic protein, termed progressin ( M r ,=33 000), has been identified in proliferating human cells of epithelial, fibroblast and lymphoid origin. Progressin is synthesized almost exclusively during the S‐phase of transformed human amnion cells (AMA). Increased synthesis of this protein is first detected late in G 1 , at or near the G 1 /S transition border, reaches a maximum in mid to late S‐phase, and declines thereafter. Contrary to histones, progressin synthesis is not coupled to DNA replication. As expected for an S‐phase‐specific protein, no detectable synthesis of progressin was observed in non‐proliferating human MRC‐5 fibroblasts and epidermal basal keratinocytes. Elevated, but variable levels of this protein were observed in proliferating normal fibroblasts and transformed cells of fibroblast, epithelial and lymphoid origin. Taken together the above observations suggest that progressin may be a component of the common pathway leading to DNA replication and cell division.