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Sequence‐dependence of the conformational changes induced by the 5‐methyl cytosine in synthetic RNA oligomers
Author(s) -
Bloch G.,
Neumann J.M.,
Babin F.,
Huynh-Dinh T.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80273-9
Subject(s) - random hexamer , cytosine , rna , sequence (biology) , chemistry , duplex (building) , stereochemistry , crystallography , biochemistry , dna , gene
The RNA hexamer containing a 5‐methyl cytosine (m5C) r(CGUAm5CG) was studied by 1 H and 31 P NMR at 500 MHz and 121 MHz, respectively. In contrast to r(CGm5CGCG) which exhibits an atypic duplex structure [(1987) J. Am. Chem. Soc. 109, 2539–2541], r(CGUAm5CG) adopts a classical A‐type conformation. This result demonstrates that the influence of the m5C on the conformation of RNA hexamers is sequence‐dependent.