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Identification of the plasma membrane receptor for interleukin‐1 on mouse thymoma cells
Author(s) -
Bron C.,
MacDonald H.R.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80254-5
Subject(s) - thymoma , receptor , identification (biology) , chemistry , microbiology and biotechnology , interleukin 2 , immunology , biophysics , biology , biochemistry , botany
The plasma membrane receptor for interleukin‐1 (IL‐1) has been characterized from mouse EL4‐6.1 thymoma cells. Following binding of IL‐1 to surface labeled EL4‐6.1 cells, the IL‐1 binding molecule was immunoprecipitated using a rabbit antiserum against the hormone. The putative IL‐1 receptor is a membrane‐associated glycopeptide of M r = 82000 containing probably two or three N‐linked glycan units as indicated by its conversion into a M r = 60 000 polypeptide upon deglycosylation with endo‐β‐ N ‐glycosidase F.