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The synergistic effect of serotonin and epinephrine on the human platelet at the level of signal transduction
Author(s) -
de Chaffoy de Courcelles D.,
Roevens P.,
Van Belle H.,
De Clerck F.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80236-3
Subject(s) - epinephrine , serotonin , signal transduction , myosin light chain kinase , intracellular , platelet , protein kinase c , chemistry , phosphorylation , endocrinology , protein kinase a , kinase , medicine , biochemistry , microbiology and biotechnology , biology , receptor
Simultaneous addition to platelets of submaximal amounts of excitatory agonists acts synergistically in provoking secretory and aggregatory responses. By measuring changes in intracellular free Ca 2+ concentration, inositol phospholipid metabolism and protein phosphorylation, we verified whether synergism could be evidenced at the level of signal transduction. Challenging platelets with epinephrine only induced minor changes on the measured parameters. However, when added together with serotonin, epinephrine amplified mobilisation of intracellular C 2+ , PA formation, PIP formation, protein kinase C and myosin light chain kinase activity as compared to the alterations induced by serotonin alone. It is concluded that synergistic effects on simultaneous addition of serotonin and epinephrine might originate at the level of signal transduction.