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Construction of a hybrid cDNA encoding a major legumin precursor polypeptide and its expression and localization in Saccharomyces cerevisiae
Author(s) -
Yarwood Jennifer N.,
Harris Nick,
Delauney Ashton,
Croy Ron R.D.,
Gatehouse John A.,
Watson Martin D.,
Boulter Donald
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80215-6
Subject(s) - legumin , saccharomyces cerevisiae , complementary dna , golgi apparatus , phosphoglycerate kinase , biochemistry , biology , microbiology and biotechnology , gene , chemistry , storage protein , endoplasmic reticulum
A hybrid cDNA encoding a major legumin precursor polypeptide was constructed. Using a phosphoglycerate kinase expression vector levels of expression of 1–2% total protein were obtained in Saccharomyces cerevisiae . Immunocytochemical localization demonstrated that the legumin was accumulated within the Golgi vesicles.