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Demonstration that LSTRA cells have an elevated level of proteins phosphorylated on tyrosine residues
Author(s) -
Thom Rebecca E.,
Casnellie John E.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80200-4
Subject(s) - tyrosine , tyrosine kinase , biology , phosphorylation , cell culture , tyrosine phosphorylation , proto oncogene tyrosine protein kinase src , protein kinase a , microbiology and biotechnology , chemistry , biochemistry , signal transduction , genetics
The LSTRA cell line has been shown to have an exceptionally high level of a tyrosine protein kinase (pp56 lck ). We now report that LSTRA cells also have a much higher level of proteins phosphorylated on tyrosine residues in comparison to several other cell lines with normal levels of pp56 lck . The level of phosphotyrosine‐containing proteins in LSTRA cells was comparable to that seen in K562 cells, a cell line known to have a constitutively active tyrosine protein kinase. These results provide evidence that LSTRA cells have an elevated level of in vivo tyrosine protein kinase activity, probably due to the overexpression and activation of pp56 lck .