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Developmental changes in the lateral diffusion of Leydig cell membranes measured by the FRAP method
Author(s) -
Zlatanov I.V.,
Foley M.,
Birmingham J.,
Garland P.B.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80189-8
Subject(s) - fluorescence recovery after photobleaching , membrane , fluorescence , photobleaching , biophysics , membrane protein , chemistry , microbiology and biotechnology , diffusion , biology , biochemistry , optics , physics , thermodynamics
A simple method for isolation and fluorescence labelling of Leydig cells (L‐cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age‐dependent changes in diffusibility of membrane lipids and proteins were discovered.