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Changes in the expression of N ‐acetylglucosaminyltransferase III, IV, V associated with the differentiation of HL‐60 cells
Author(s) -
Koenderman Anky H.L.,
Wijermans Pierre W.,
van den Eijnden Dirk H.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80188-6
Subject(s) - transferase , retinoic acid , myeloid , cellular differentiation , microbiology and biotechnology , biology , chemistry , biochemistry , enzyme , immunology , gene
By use of a new assay method based on HPLC, GlcNAc‐transferase III, IV and V activities were determined in HL‐60 cells, differentiated HL‐60 cells and normal myeloid cells. Differentiation along the monocytic lineage with 1α,25‐dihydroxyvitamin D 3 resulted in increased GlcNAc‐transferase IV and decreased GlcNAc‐transferase III activity. Differentiation along the myeloid lineage with retinoic acid resulted in a decrease in GlcNAc‐transferase III activity. Although differentiated HL‐60 cells show a changed GlcNAc‐transferase pattern, they do not resemble normal myeloid cells. Macrophages and granulocytes are characterized by a very low level of GlcNAc‐transferase III activity whereas differentiated HL‐60 cells still contain this activity. This is the first demonstration of GlcNAc‐transferase IV and V activity in a human cell.