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Antigenic relatedness between phospholipases A 2 from Naja naja venom and from mammalian cells
Author(s) -
Masliah J.,
Kadiri C.,
Pepin D.,
Rybkine T.,
Etienne J.,
Chambaz J.,
Bereziat G.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80182-5
Subject(s) - polyclonal antibodies , venom , antiserum , naja , guinea pig , antibody , antigen , biology , microbiology and biotechnology , epitope , extracellular , phospholipase a , phospholipase , biochemistry , phospholipase a2 , immunology , enzyme , endocrinology
Polyclonal antiserum was prepared against phospholipase A 2 from Naja naja and used to prepare a purified antibody. It cross‐reacted with the antigen, and with intracellular mammalian PLA 2 . This antibody was immunoreactive and inhibited the PLA 2 activity of Naja naja and of guinea pig alveolar macrophages or rat lymphocytes. By immunoblotting, this antiserum revealed one band of PLA 2 from Naja naja (14 kDa) and 3 bands for guinea pig alveolar macrophages and rat lymphocytes (30, 45 kDa and a minor band of 14 kDa). These results show an antigenic relatedness between an extracellular PLA 2 and membrane‐bound PLA 2 from two different mammalian species and cell types.