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Differential effects of DNA tumor virus nuclear oncogene products on adipocyte differentiation
Author(s) -
Tobe Kazuyuki,
Kasuga Masato,
Kitasato Hidero,
Takaku Fumimaro,
Takano Toshiya,
Segawa Kaoru
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80175-8
Subject(s) - oil red o , adipogenesis , adipocyte , oncogene , polyoma virus , lipid droplet , inducer , staining , biology , microbiology and biotechnology , dna , antigen , gene , cell , cellular differentiation , chemistry , adipose tissue , biochemistry , cell cycle , immunology , genetics
We have introduced SV40 and polyoma large T antigen‐ and adenovirus‐type 12 E1A genes into mouse 3T3‐L1 preadipocyte cells to study the ability of various nuclear oncogene products to modulate cell differentiation. Clones expressing E1A products could differentiate into adipocytes faster than the control in spite of the absence of adipogenic inducers, as measured by the appearance of lipid droplets microscopically and by staining accumulated triglycerides with oil red O. However, clones expressing SV40 and polyoma large T antigens could not differentiate even if they were exposed to the inducers.