Premium
Conjugation‐dependent recovery of the Na + pump in a mutant of Vibrio alginolyticus lacking three subunits of the Na + pump
Author(s) -
Tokuda Hajime,
Udagawa Toshiaki,
Asano Makoto,
Yamamoto Tomoko,
Unemoto Tsutomu
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80173-4
Subject(s) - vibrio alginolyticus , mutant , plasmid , wild type , chemistry , protein subunit , vibrio , biology , biochemistry , bacteria , gene , genetics
The Na + pump‐deficient mutant, Napl, of Vibrio alginolyticus was found to lack three subunits of Na + ‐dependent NADH:quinone oxidoreductase complex. Although a spontaneous Na + pump positive revertant did not appear from Napl, transconjugants that recovered both the Na + pump activity and the subunits were isolated from Napl conjugated with the wild type. Moreover, the wild type was found to contain two different sizes of plasmids. These results suggest the possibility that the Na + pump is encoded by a plasmid.