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The quantitation of biotinylated compounds by a solid‐phase assay using a 125 I‐labelled biotin derivative
Author(s) -
Smith Peter J.,
Warren Robin M.,
von Holt Claus
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80167-9
Subject(s) - biotinylation , biotin , derivative (finance) , chemistry , phase (matter) , chromatography , biochemistry , organic chemistry , financial economics , economics
The biotin analogue biotinylglycyltyrosine has been synthesized and labelled to a specific activity of 2000 Ci/mmol with 125 I. This analogue has been used in conjunction with immobilized streptavidin in an assay which detects as little as 1 fmol biotin or biotinylated molecules in solution. The determination of biotinylated insulin in a tissue extract and the quantitation of a transcription assay are given as examples.