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Nuclear Overhauser effects in linear peptides A low‐temperature 500 MHz study of Met‐enkephalin
Author(s) -
Motta A.,
Tancredi T.,
Temussi P.A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80148-5
Subject(s) - nuclear overhauser effect , chemistry , enkephalin , nuclear magnetic resonance , biophysics , nuclear magnetic resonance spectroscopy , stereochemistry , receptor , biology , biochemistry , physics , opioid
Met 5 ‐enkephalin was studied in 1 mM solutions in 2 H 2 O at room temperature and in a cryoprotective mixture (DMSO d6 / 2 H 2 O, mole fraction of DMSO 0.49) in the temperature range 265–298 K. Small positive effects were observed between the ortho and meta protons of Tyr in aqueous solution at room temperature. Intraresidue effects can be made strong and negative by increasing the viscosity of the medium with a combination of cryoprotective mixtures and low temperatures. The use of mixtures with properties very close to water is very promising for conformational studies of enkephalins and of other small linear peptides.