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Glucose stimulates the biosynthesis of rat I and II insulin to an equal extent in isolated pancreatic islets
Author(s) -
Rhodes Christopher J.,
Lucas Christine A.,
Halban Philippe A.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80137-0
Subject(s) - proinsulin , insulin , medicine , biosynthesis , endocrinology , pancreatic islets , islet , chemistry , methionine , biology , amino acid , biochemistry , enzyme
The effects of glucose on insulin biosynthesis were studied by measuring the incorporation of radiolabelled amino acids into proinsulin/insulin in isolated rat islets. The islets were pulse labelled for 15 min with [ 3 H]leucine (present in rat insulin I and II) or [ 35 S]methionine (unique to rat insulin II) and then incubated for a 165 min post‐label (chase) period during which the majority of labelled proinsulin was converted to insulin but under conditions whereby > 95% of radiolabelled proinsulin or insulin was retained in the islets. The newly synthesized, labelled, insulin was analyzed by high performance liquid chromatography. Rat I and II insulin biosynthesis was stimulated by 16.7 mM glucose to the same extent.