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Mucin‐carbohydrate directed monoclonal antibody
Author(s) -
Kurosaka Akira,
Fukui Shigeyuki,
Kitagawa Hiroshi,
Nakada Hiroshi,
Numata Yoshito,
Funakoshi Ikuo,
Kawasaki Toshisuke,
Yamashina Ikuo
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80128-x
Subject(s) - mucin , monoclonal antibody , carbohydrate , chemistry , biochemistry , antibody , biology , immunology
To raise monoclonal antibodies recognizing cancer‐associated alterations of the carbohydrate structure of glycoproteins, Balb/c mice were immunized with human colonic cancer cells (LS 180 from ATCC). One of the generated hybridomas produced a monoclonal antibody that bound to the carbohydrate moiety of mucin‐type glycoproteins from LS 180. The antibody did not bind to glycoproteins from another colonic cancer cell line, SW 1116, or to glycolipids from any of the colonic cancer cell lines. The antibody bound to ovine and bovine submaxillary mucins (OSM and BSM). NeuAcα2→6Ga1NAc seemed to be involved in the epitope.