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Isolation and characterization of a rubredoxin and a flavodoxin from Desulfovibrio desulfuricans Berre‐Eau
Author(s) -
Fauque Guy D.,
Moura Isabel,
Moura José J.G.,
Xavier António V.,
Galliano Nicole,
LeGall Jean
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80114-x
Subject(s) - flavodoxin , rubredoxin , hydrogenase , desulfovibrio , chemistry , electron transfer , electron acceptor , biochemistry , crystallography , ferredoxin , photochemistry , enzyme , sulfate , organic chemistry
A rubredoxin and a flavodoxin have been purified and characterized from soluble extracts of a sulfate‐reducing bacterium able to grow with N 2 as the only nitrogen source: Desulfovibrio desulfuricans strain Berre‐Eau. These two electron carriers have characteristics similar to homologous proteins found in other Desulfovibrio species (molecular mass, absorption spectrum, extinction coefficient and amino acid composition). In contrast to rubredoxin, flavodoxin mediates electron transfer in the reduction of sulfite to sulfide and in hydrogen evolution from pyruvate, when in the presence of hydrogenase