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Photoinhibition: Impairment of the primary charge separation between P‐680 and pheophytin in photosystem II of chloroplasts
Author(s) -
Demeter Sandor,
Neale Patrick J.,
Melis Anastasios
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80090-x
Subject(s) - pheophytin , photoinhibition , chlamydomonas reinhardtii , photosystem ii , photochemistry , chemistry , chloroplast , biophysics , biology , photosynthesis , biochemistry , mutant , gene
The question of the primary site of photoinhibition was investigated using the light‐induced absorbance change of pheophytin a (at 685 nm) in spinach chloroplasts and Chlamydomonas reinhardtii cells. Photoinhibition of spinach thylakoid membranes resulted in a parallel decrease in the amplitude of the pheophytin a (Δ A 685 ) and Q A (Δ A 320 ) photoreduction signals. In intact Chlamydomonas reinhardtii cells the pheophytin photoreduction and oxygen evolution activity exhibited a similar decrease during photoinhibition. A complete recovery of both activities was attained within 60 min incubation in normal growth conditions. It is concluded that the primary site of photoinhibition involves the components (P‐680 and/or pheophytin) of the primary charge separation.