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A method for removal of trace iron contamination from biological buffers
Author(s) -
Gutteridge John M.C.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80088-1
Subject(s) - reagent , contamination , transferrin , chemistry , dialysis , chromatography , environmental chemistry , radiochemistry , biochemistry , organic chemistry , biology , surgery , medicine , ecology
Laboratory chemicals and reagents normally contain trace amounts of iron salts sufficient to catalyse free radical reactions. This iron contamination can be removed from buffers and reagents using a dialysis sac containing a high‐affinity iron‐binding protein like conalbumin or transferrin without altering the pH value of the fluid.