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The human insulin‐like growth factor II gene contains two development‐specific promoters
Author(s) -
de Pagter-Holthuizen P.,
Jansen M.,
van Schaik F.M.A.,
van der Kammen R.,
Oosterwijk C.,
Van den Brande J.L.,
Sussenbach J.S.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80066-2
Subject(s) - promoter , exon , gene , biology , complementary dna , insulin like growth factor 2 , insulin like growth factor , genetics , human insulin , microbiology and biotechnology , gene expression , growth factor , insulin , endocrinology , receptor
The insulin‐like growth factors (IGF) play an important role in fetal and postnatal development. Recently, the nucleotide sequences of the cDNAs encoding IGF‐I and IGF‐II and part of the human IGF genes were reported. In this communication we describe two distinct IGF‐II cDNAs isolated from a human adult liver and a human hepatoma cDNA library, respectively. Using these two cDNAs, we have established that the human IGF‐II gene contains at least 7 exons. Two different IGF‐II promoters have been identified, 19 kilobases (kb) apart, which are active in a development‐specific manner. The promoter, active in the adult stage, is located only 1.4 kb downstream from the insulin gene.