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Differentiation of creatine kinase MB and IgA‐linked BB isoenzymes on electrophoresis
Author(s) -
Chen Ann,
Wong Shan S.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80040-6
Subject(s) - isozyme , chemistry , creatine kinase , electrophoresis , gel electrophoresis , microbiology and biotechnology , biochemistry , enzyme , biology
IgA‐linked creatine kinase (CK, EC 2.7.3.2) is a macro CK type 1 isoenzyme that has an identical electrophoretic mobility to CK‐MB. Its presence has the potential of causing misdiagnosis of myocardial infarction. Mixing anti‐CK‐B antiserum with the sample prior to electrophoresis did not unequivocally distinguish between the two isoenzymes. Similarly, anti‐human IgG and IgM antibodies were also ineffective. However, the IgA‐linked isoenzyme band was removed by anti‐human IgA antiserum. While anti‐CK‐M antibodies did not affect the electrophoretic mobility of IgA‐linked CK‐BB, the antibody eliminated both the CK‐MB and CK‐MM bands. Thus, specific anti‐IgA and anti‐CK‐M antibodies may be used to establish the presence of the myocardial isoenzyme.