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Stimulus‐response coupling in a cell‐free platelet membrane system
Author(s) -
O'Rourke Flavia,
Zavoico George B.,
Smith Laurens H.,
Feinstein Maurice B.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80037-6
Subject(s) - stimulus (psychology) , chemistry , platelet , biophysics , membrane , neuroscience , biology , psychology , biochemistry , immunology , psychotherapist
The Ca 2+ ‐mobilizing action of thrombin was demonstrated in a cell‐free platelet membrane system consisting of open sheets of plasma membrane plus sealed membrane vesicles that accumulate Ca 2+ and release Ca 2+ in response to IP 3 . Thrombin plus GTP, acting on plasma membrane (not vesicles), produced a soluble factor (destroyed by alkaline phosphatase) that released Ca 2+ from the vesicles. This effect of thrombin/GTP was blocked by a monoclonal antibody that binds to vesicles and prevents Ca 2+ release by IP 3 . Pertussis toxin plus NAD ADP‐ribosylated plasma membrane polypeptides of 39 and 41 kDa and blocked Ca 2+ release by thrombin/GTP, but not by IP 3 .