Premium
The effects of protein synthesis inhibition, and of mutations rna1.1 and rna82.1 , on the synthesis of small RNAs in yeast
Author(s) -
Piper Peter W.,
Lockheart Alan,
Bellatin Jaime
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80030-3
Subject(s) - rna , rna splicing , transfer rna , ribosomal rna , biology , saccharomyces cerevisiae , endonuclease , 5.8s ribosomal rna , biochemistry , messenger rna , non coding rna , yeast , protein biosynthesis , microbiology and biotechnology , genetics , enzyme , gene
Strains of Saccharomyces cerevisiae have been constructed that possess temperature‐sensitive defects in tRNA precursor (pre‐tRNA) splicing and which also lack the processing endonuclease that acts at the 3′‐terminus of 5 S rRNA and 35 S rRNA precursors (pre‐rRNAs). The unspliced pre‐tRNAs accumulated by such strains at the nonpermissive temperature are identical in structure to those accumulated by pre‐tRNA splicing‐defective strains with a functional pre‐5 S RNA processing enzyme. The pre‐RNA processing activity is therefore not obligatorily involved in maturation of several yeast tRNAs. However, gels of the pulse‐labelled RNAs of RNA82 + and rna82.1 strains provide evidence that this enzyme acts upon a few small unstable transcripts that are not 5 S RNA forms. The most prominent of these transcripts on gels was, in wild‐type strains, an RNA 145 ± 2 nucleotides in length.