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Brefeldin A arrests the intracellular transport of a precursor of complement C3 before its conversion site in rat hepatocytes
Author(s) -
Oda Kimimitsu,
Hirose Shinichi,
Takami Noboru,
Misumi Yoshio,
Takatsuki Akira,
Ikehara Yukio
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80028-5
Subject(s) - brefeldin a , monensin , golgi apparatus , secretion , intracellular , intracellular transport , microbiology and biotechnology , chemistry , hepatocyte , chromosomal translocation , biochemistry , biology , in vitro , cell , gene
The effects of brefeldin A on intracellular transport and posttranslational modification of complement C3 (C3) were studied in primary culture of rat hepatocytes. In the control culture C3 was synthesized as a pre‐cursor (pro‐C3), which was processed to the mature form with α‐ and β‐subunits before its discharge into the medium. In the presence of brefeldin A the secretion of C3 was strongly blocked, resulting in accumulation of pro‐C3. However, after a prolonged interval the mature form of C3 was finally secreted. The results indicate that brefeldin A impedes translocation of pro‐C3 to the Golgi complex where pro‐C3 is converted to the mature form, but not its proteolytic processing, in contrast to the effects of monensin and weakly basic amines.