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Disulfide bonds within the α‐subunit of insulin receptors in rat liver and brain membranes
Author(s) -
Nagao Munehiko,
Sakamoto Choitsu,
Matozaki Takashi,
Baba Shigeaki
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80022-4
Subject(s) - protein subunit , disulfide bond , receptor , membrane , insulin , chemistry , insulin receptor , biochemistry , medicine , endocrinology , biophysics , biology , insulin resistance , gene
We have characterized inter‐ and intrasubunit disulfide bonds of insulin receptors using reductant‐treated rat liver and brain membranes. In autoradiograms of 125 I‐insulin cross‐linked to both membranes pretreated with dithiothreitol, the intensity of affinity‐labeled bands of the αβ‐heterodimer and α‐subunit was increased. Interestingly, labeled 120 and 110 kDa bands considered to be the α‐subunit in partially reduced liver and brain membranes moved to 130 and 120 kDa bands under further reduced conditions, respectively. Double electrophoresis of each partially reduced band in the presence of reductants clearly demonstrates that the α‐subunit of insulin receptors contains intrasubunit disulfide bonds.