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Interaction of chlorophyll a ′ with the 65 kDa subunit protein of photosystem I reaction center
Author(s) -
Hiyama Tetsuo,
Watanabe Tadashi,
Kobayashi Masami,
Nakazato Masataka
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80020-0
Subject(s) - p700 , photosystem i , absorbance , photosynthetic reaction centre , light harvesting complexes of green plants , photosystem ii , chlorophyll a , chemistry , chlorophyll , protein subunit , photochemistry , spectral line , analytical chemistry (journal) , photosynthesis , biochemistry , physics , chromatography , electron transfer , organic chemistry , astronomy , gene
The 65 kDa polypeptide subunit depleted of P700 was prepared from a photosystem I reaction center preparation and mixed with chlorophyll a ′ (C‐10 epimer of chlorophyll a ) to yield a complex exhibiting a tripleheaded spectrum with absorbance maxima at 673, 692 and 707 nm. The difference spectra (oxidized‐minus‐untreated and light‐minus‐dark) had a major trough at 707 nm and minor ones at 690 and 430 nm. The overall shape of the spectra resembled well that of P700 with a small red shift. A rapidly decaying flash‐induced absorbance change was observed at 430 nm with a half decay time of less than 500 μs in a preparation supplemented with an electron donor system.