Premium
Expression of a truncated v‐myb product in transformed chicken embryo fibroblasts
Author(s) -
Kryceve-Martinerie Cécile,
Soret Johann,
Crochet Janine,
Baluda Marcel,
Perbal Bernard
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80017-0
Subject(s) - myb , clone (java method) , biology , transfection , recombinant dna , embryo , microbiology and biotechnology , transformation (genetics) , dna , cell culture , gene , genetics , gene expression
Transformed cells have been isolated after transfection of chicken embryo fibroblasts (CEF) with the DNA of a recombinant clone (KXA 3457) in which the v‐myb sequences are flanked by the two AMV‐LTRs. Abnormal myb ‐specific RNA species and myb ‐related polypeptides were found to be expressed in these cells, suggesting that transformation of CEF by v‐myb might require alterations of the oncogene product.