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Homology between bacterial DNA and bovine mitochondrial DNA encoding cytochrome c oxidase subunit III
Author(s) -
Fink Pamela S.,
Whitford Tracy,
Leffak Michael,
Prochaska Lawrence J.
Publication year - 1987
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(87)80016-9
Subject(s) - mitochondrial dna , cytochrome c oxidase , protein subunit , cytochrome b , homology (biology) , dna , nadh dehydrogenase , biology , microbiology and biotechnology , biochemistry , chemistry , genetics , gene , mitochondrion
A segment of mitochondrial DNA encoding the bovine cytochrome c oxidase subunit III gene was isolated and inserted into an Escherichia coli plasmid vector. A 556 base pair fragment of the insert DNA representing about 70% of the 3′‐end of the subunit III gene was used to search for homology with bacterial DNA from strains that contain heme aa 3 ‐type cytochrome c oxidases. Bacillus subtilis, Thermus thermophilus, and PS3 DNAs all showed strong hybridization to the probe, whereas Paracoccus denitrificans and Rhodopseudomonas sphaeroides DNAs showed only weak hybridization to the probe, even under low stringency conditions.