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Adenine nucleotide compartmentation in foetal rat hepatocytes
Author(s) -
Roncero César,
Estrela José M.,
Benito Manuel
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81541-1
Subject(s) - nucleotide , chemistry , adenine nucleotide , biochemistry , biology , gene
In the presence of lactate plus pyruvate, or glucose or alanine as substrates, ATP/ADP ratios in the cytosol were higher than in mitochondria in isolated rat foetal hepatocytes. The cytosolic ATP/ADP ratios were dependent on substrate (lactate + pyruvate > glucose > alanine). Oleate increased the cytosolic ATP/ADP ratios in the presence of the other substrates studied. Atractyloside decreased the cytosolic ATP/ADP ratios, oligomycin decreasing these values in both compartments. Isoproterenol, phenylephrine and Ca 2+ ionophore decreased the cytosolic ATP/ADP ratio, without altering this value in mitochondria.