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Assignment of the 31 P and 1 H resonances in oligonucleotides by two‐dimensional NMR spectroscopy
Author(s) -
Sklenár̆ Vladimir,
Miyashiro Hirotsugu,
Zon Gerald,
Todd Miles H.,
Bax Ad
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81539-3
Subject(s) - heteronuclear molecule , oligonucleotide , spectroscopy , resonance (particle physics) , chemistry , sequence (biology) , nuclear magnetic resonance spectroscopy , crystallography , dna , physics , nuclear magnetic resonance , stereochemistry , atomic physics , biochemistry , quantum mechanics
The use of new 1 H‐detected heteronuclear 1 H‐ 31 P shift correlation experiments is demonstrated for oligonucleotides of 12 and 40 base pairs. The methods give unambiguous assignments of the 31 P resonances and also permit identification of the C4′ and C5′ sugar protons. Use of the new methods enables one to make sequence‐specific resonance assignments without reference to a known or assumed conformation of the DNA fragment.