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Sliding‐end‐labelling
Author(s) -
Pérez-Ortin Josh E.,
Estruch Francisco,
Matallana Emilia,
Franco Luis
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81525-3
Subject(s) - labelling , nucleosome , nuclease , dna , chemistry , biophysics , biochemistry , biology , histone
A method, termed ‘sliding‐end‐labelling’, has been devised to avoid a frequent artifact in nucleosome positioning by indirect end labelling, namely the appearing of DNA fragments originated by two nuclease cuts, one of them lying within the region covered by the probe. The method is applied to the nucleosome positioning in the yeast SUC2 gene for invertase.