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A peculiar repetitive sequence in the rat genome
Author(s) -
Nojima Hiroshi,
Sokabe Hirofumi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81493-4
Subject(s) - sequence (biology) , genetics , computational biology , genome , biology , whole genome sequencing , gene
We report here a new type of peculiar repetitive sequence, A 15 T(TC) 9 T 12 , which was detected at 750 base pairs (bp) upstream of a rat calmodulin processed pseudogene by DNA sequencing of cloned DNA fragments. This sequence element could possibly form a cruciform structure with a 12‐AT‐pair stem, exposing (CT) 9 sequences as a loop. S 1 nuclease protection experiments failed to identify this element as a cruciform structure but instead detected an alternating purine pyrimidine tract at 50 bp downstream of this element. Total genomic Southern blotting showed that the rat genome contains only a few of these elements.