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Enzymic removal of two oligosaccharide chains from ricin B‐chain
Author(s) -
Wawrzynczak Edward J.,
Thorpe Philip E.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81490-9
Subject(s) - ricin , oligosaccharide , chemistry , biochemistry , asparagine , lactose , chain (unit) , protein subunit , enzyme , toxin , physics , astronomy , gene
Peptide: N ‐glycosidase F removed both the asparagine‐linked oligosaccharide chains of ricin B‐chain in the absence of lactose. In the presence of lactose, which binds specifically to the B‐chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B‐chain against the removal of one of the two susceptible oligosaccharides present in each B‐chain subunit.