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β‐Subunits of the human liver G s /G i signal‐transducing proteins and those of bovine retinal rod cell transducin are identical
Author(s) -
Codina Juan,
Stengel Dominique,
Woo Savio L.C.,
Birnbaumer L.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81486-7
Subject(s) - transducin , complementary dna , open reading frame , microbiology and biotechnology , biology , stop codon , polyadenylation , cdna library , nucleotide , nucleic acid sequence , untranslated region , start codon , peptide sequence , signal peptide , amino acid , biochemistry , g protein , messenger rna , dna , gene , signal transduction
The complete cDNA encoding the β‐subunit of the human liver signal transducing proteins G s /G i (β G ) has been cloned from a λgtll library using an oligonucleotide as a screening agent. The cDNA has 3088 nucleotides and an 11 nucleotide poly(A) tail, of which 280 nucleotides constitute the 5′‐untranslated region, 1023 form the open reading frame (ORF) and its stop codon, and 1785 are the 3′‐untranslated region with two AATAAA cleavage and polyadenylation signals separated by 1467 nucleotides. The ORF codes for a 340 amino acid polypeptide that is identical to that encoded by bovine retinal rod cell cDNA of the β‐subunit of transducin. Yet, it does so by using 87 different codons. Curiously, the 280 nucleotide 5′ leader sequence obtained starts with an ATG that is part of another ORF encoding a putative peptide X of 75 amino acids (nucleotide 280 to 55). This work proves for the first time that the β‐subunits of all signal‐transducing G‐proteins, including transducin, are the same.