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The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli
Author(s) -
Meier N.,
Göringer H.U.,
Kleuvers B.,
Scheibe U.,
Eberle J.,
Szymkowiak C.,
Zacharias M.,
Wagner R.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81392-8
Subject(s) - nucleotide , function (biology) , chemistry , ribosomal rna , molecule , computational biology , biochemistry , biology , genetics , gene , organic chemistry
Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of denned nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide‐directed or bisulfite‐catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA‐protein interaction assembly and mRNA‐coded tRNA binding.