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Flow‐force relationships in mitochondrial oxidative phosphorylation
Author(s) -
Woelders Henri,
Putters Jacques,
van Dam Karel
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81379-5
Subject(s) - valinomycin , oxidative phosphorylation , phosphorylation , chemistry , mitochondrion , membrane potential , biophysics , membrane , uncoupling agents , biochemistry , biology
The rates of oxidation and phosphorylation in isolated rat‐liver mitochondria have a steep dependence on the protonmotive force (Δ≈sm h ) across the membrane. These experimentally observed relationships proved to be independent of the way in which δ≈gm h was varied. These results were obtained when the membrane potential (ΔΨ) was calculated from the distribution of K + (in the presence of valinomycin). When triphenyl‐methylphosphonium (TPMP + ) was used as a probe for ΔΨ, slightly different flow‐force relationships were obtained. We conclude that unique relationships exist between Δ≈sm h + and the rates of oxidation and phosphorylation, and that under some conditions the behaviour of the probe TPMP + is anomalous.