Premium
Mapping of HLA genes using pulsed‐field gradient electrophoresis
Author(s) -
Ragoussis Jiannis,
van der Bliek Alexander,
Trowsdale John,
Ziegler Andreas
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81376-x
Subject(s) - pulsed field gel electrophoresis , linkage (software) , gene , genetics , human leukocyte antigen , major histocompatibility complex , genetic linkage , biology , microbiology and biotechnology , gel electrophoresis , computational biology , antigen , genotype
The technique of pulsed‐field gradient electrophoresis (PFGE) allows the determination of gene linkage relationships since DNA fragments up to 2 Mb can be separated. PFGE was employed to study linkage of class I, II and III genes belonging to the human major histocompatibility (HLA) complex. The results establish that the class II DOβ and DZα genes are linked with the DP Subregion, centromeric to the DQ/DX‐DR‐C4 chromosomal segment, and allow us to estimate the minimal length of the entire HLA complex.