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Ca 2+ and phorbol ester synergistically induce HL‐60 differentiation
Author(s) -
Tyers Michael,
Harley Calvin B.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81348-5
Subject(s) - ionophore , phorbol ester , phorbol , chemistry , cellular differentiation , monocyte , granulocyte , biochemistry , microbiology and biotechnology , enzyme , medicine , biology , immunology , protein kinase c , membrane , gene
Exposure of HL‐60 cells to subthreshold concentrations of TPA caused monocytic differentiation only when cells were cotreated with the Ca 2+ ionophore A23187. Phorbol ester dose‐response curves for growth arrest and enzymatic markers of differentiation were shifted to lower concentrations when the ionophore was present. Expression of a monocyte/granulocyte cell surface antigen also occurred only when cells were treated with both agents. Similar effects were seen with other active but not inactive phorbol esters and with another Ca 2+ ionophore. The Ca 2+ component of phosphoinositide‐based signalling may thus play a role in HL‐60 differentiation.